RNA-sequencing of in vitro differentiated WT and Chi3l1 KO Th1 cell
We report the differential mRNA expressino of WT and Chi3l1 KO Th1 cells. We cultured WT and Chi3l1 KO naive CD4 T cells under Th1 skewing condition : plate-bound anti-CD3/28 antibody (2ug/mL), IL-12 0.2ng/mL, IL-2 50U/mL, anti-IL-4 neutralizing antibody 2ug/mL, for 3 days. We found different Th1 regulatory and tumoricidal-related gene expression in Chi3l1 KO T cells.
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytot... 2018 · 153 cites
Deep data QC
100/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Bulk RNA-seq (mouse). Grade A: exceptionally low PCR duplication (4.59%) and high base quality (Q30=96.7%, base quality=38.2) indicate excellent library quality and sequencing. Minimal adapter content (2.48%) is easily trimmed if needed. Long reads (101 bp) provide good sequence complexity. Top-tier dataset for all RNA-seq applications.
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0