Evidence for rRNA 2'-O-methylation plasticity: control of intrinsic translational capabilities of human ribosomes
Ribosomal RNAs (rRNAs) are main effectors of mRNA decoding, peptide-bond formation and ribosome dynamics during translation. Ribose 2'-O-methylation (2'-O-Me) is the most abundant rRNA chemical modification, and display a complex pattern in rRNA. 2'-O-Me was shown to be essential for accurate and efficient protein synthesis in eukaryotic cells. However, whether rRNA 2'-O-Me is an adjustable feature of the human ribosome and a means of regulating ribosome function remains to be determined. Here w...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deep data QC
97/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Human bulk RNA-seq. A-grade (97/100) driven down by high PCR duplication (37.75%, scored 83/100), a common issue in RNA-seq but risking inflated transcript abundance from duplicated fragments. High base quality (Q30=90.3%) compensates; safe for discovery but quantitative claims should note duplication caveat. Fetch error prevented metadata retrieval but all sequence metrics measured.
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0