Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE105773

GEO first seen 2018

RNA editing in nascent RNA affects pre-mRNA splicing

Organism
Homo sapiens
Samples
12
Type
Expression profiling by high...
Submitted
2017-10-23

In eukaryotes, nascent RNA transcripts undergo an intricate series of RNA processing steps to achieve mRNA maturation. RNA editing and alternative splicing are two major RNA processing steps that can introduce significant modifications to the final gene products. By tackling these processes in isolation, recent studies have enabled substantial progress in understanding their global RNA targets and regulatory pathways. However, the interplay between individual steps of RNA processing, an essentia...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Yun-Hua E HsiaoXinshu Xiao

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human bulk RNA-seq. Grade-B failure (85/100) driven by extreme PCR duplication (51.62%, scored 52/100) and poor base quality (Q30=86.5%, scored 82/100), both measured. Duplication at this level creates substantial quantification bias; not recommended for expression quantification or differential analyses. Fetch error also prevented metadata validation.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.04 measured
pct q20 bases 94.2 measured
pct q30 bases 86.5 measured
gc content pct 52.1 measured
mean read length 101 measured
mean base quality 34.6 measured
adapter content pct 0.24 measured
duplication rate pct 51.62 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 86.5 measured ×1 83%
mean base quality 34.6 measured ×0.6 100%
adapter content pct 0.24 measured ×0.4 100%
duplication rate pct 51.62 measured ×0.4 52%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0