Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE106138

GEO first seen 2021

Transcriptional regulation of adaptive NK and CD8 T cell responses [RNA-Seq]

Organism
Mus musculus
Samples
88
Type
Expression profiling by high...
Submitted
2017-10-25

Natural killer (NK) cells are innate lymphocytes that possess features of adaptive immunity such as clonal expansion and generation of long-lived memory. Here we look at transcriptional profiles of NK cells throughout several time points during MCMV infection, ex-vivo cytokine stimulation, and/or deficiency of key transcription factors such as STAT4, STAT1, and Runx1. In addition, we profile parallel time points of MCMV-specific CD8 T cells during infection and memory formation.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Colleen M LauJoseph C Sun
Reused by

Deep data QC

88/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Mouse bulk RNA-seq (HiSeq 2500). Grade-B data (88/100) limited by Q30=84.4% (scored 72/100), below ideal thresholds for isoform detection, measured. Minimal duplication (6.29%) and large depth (4B bases) provide partial compensation; suitable for discovery but not recommended for high-sensitivity variant or isoform calls.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
88 / 88 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 409095589700 reported
total reads 4090955897 reported
n content pct 0.004 measured
pct q20 bases 91.3 measured
pct q30 bases 84.4 measured
gc content pct 48 measured
mean read length 50 measured
mean base quality 34.3 measured
adapter content pct 0.04 measured
duplication rate pct 6.29 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 88/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.4 measured ×1 72%
mean base quality 34.3 measured ×0.6 100%
adapter content pct 0.04 measured ×0.4 100%
duplication rate pct 6.29 measured ×0.4 100%
QC cost 11 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0