Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE106140

GEO first seen 2018

Dynamic remodeling of translation programs drives hepatocyte proliferation during liver regeneration

Organism
Mus musculus
Samples
16
Type
Expression profiling by high...
Submitted
2017-10-25

During liver regeneration, most new hepatocytes arise from pre-existing ones; yet, the underlying mechanisms that drive these cells from quiescence to proliferation remain poorly defined. By using high-resolution transcriptome profiling of polysome fractions from purified hepatocytes isolated from quiescent and toxin-injured adult mouse livers,we uncover the mRNA transcripts regulated during liver regeneration. The translational remodeling modulates protein levels of a set of splicing factors in...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Auinash KalsotraSushant BangruWaqar Arif
Reused by

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (mouse). Grade B: high base quality (Q30=97.2%, base quality=39.7) undermined by severe PCR duplication (68.2%), suggesting library complexity or amplification issues. The long reads (99.5 bp mean) are favorable, but elevated duplication will inflate variance in expression estimates for low-abundance genes. Consider filtering duplicates or using UMI-aware quantitation if available.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.003 measured
pct q20 bases 99.1 measured
pct q30 bases 97.2 measured
gc content pct 47.3 measured
mean read length 99.5 measured
mean base quality 39.7 measured
adapter content pct 0 measured
duplication rate pct 68.18 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.2 measured ×1 100%
mean base quality 39.7 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 68.18 measured ×0.4 15%
QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0