Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE106210

GEO first seen 2018

Transcriptomic response to adult hypothalamic deletion of Snord116

Organism
Mus musculus
Samples
9
Type
Expression profiling by high...
Submitted
2017-10-26

Snord116 deletion mouse models recapitulate aspects of the Prader-Willi Syndrome. In this study, we examine the gene expression changes in the mediobasal hypothalamus for mice which have an adult onset deletion of Snord116 in the mediobasal hypothalamus.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Joseph Polex-WolfBrian Y LamAnthony P CollStephen O'RahillyGiles S Yeo
Reused by

Deep data QC

90/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (mouse). Grade A: high base quality (Q30=96.7%, base quality=39.3) offset by moderate-to-high PCR duplication (56.2%). While above the B threshold, duplication at this level will noticeably increase noise in abundance estimates, especially for minor transcripts. Usable for robust genes but recommend duplicate-aware analysis or validation for low-abundance targets.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.008 measured
pct q20 bases 99 measured
pct q30 bases 96.7 measured
gc content pct 56.5 measured
mean read length 50 measured
mean base quality 39.3 measured
adapter content pct 0.51 measured
duplication rate pct 56.2 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 90/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.7 measured ×1 100%
mean base quality 39.3 measured ×0.6 100%
adapter content pct 0.51 measured ×0.4 100%
duplication rate pct 56.2 measured ×0.4 42%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0