Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE106368

GEO first seen 2018

Cooperation of GRSF1 and the mitochondrial degradosome (hSuv3-PNPase complex) in degradation of mitochondrial RNA

Organism
Homo sapiens
Samples
41
Type
Expression profiling by high...
Submitted
2017-10-31

Vertebrate mitochondrial genomes show extraordinary GC skew which results in the synthesis of RNAs prone to form G-quadruplexes (G4). Such RNAs, although mostly non-coding, are transcribed at high rate and swiftly degraded by an unknown mechanism. Comprehensive proteomic and transcriptomic approaches revealed that quasi-RRM protein GRSF1 together with the mitochondrial degradosome composed of RNA helicase hSuv3 and PNPase control the levels of G4 containing RNAs in mitochondria. When this machin...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Zbigniew PietrasTomasz M KulinskiAndrzej DziembowskiRoman J Szczesny
Reused by

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (human). Grade B: high base quality (Q30=92.8%, base quality=36) and large sample size (528M reads, 80B bases) are negated by extreme PCR duplication (96.93%), indicating near-clonal amplification and severe library representation bias. This dataset is largely unsuitable for expression quantitation without deep investigation and extensive filtering.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
41 / 41 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 80346483168 reported
total reads 528595284 reported
n content pct 0.001 measured
pct q20 bases 97.9 measured
pct q30 bases 92.8 measured
gc content pct 43.7 measured
mean read length 76 measured
mean base quality 36 measured
adapter content pct 0 measured
duplication rate pct 96.93 measured
supplementary file types BW, CSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.8 measured ×1 100%
mean base quality 36 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 96.93 measured ×0.4 0%
QC cost 6 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0