Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE107011

GEO first seen 2019

RNA-Seq profiling of 29 immune cell types and peripheral blood mononuclear cells

Organism
Homo sapiens
Samples
127
Type
Expression profiling by high...
Submitted
2017-11-16

We performed RNA-Seq transcriptome profiling on 29 immune cell types consituting peripheral blood mononuclear cells (PBMCs) sorted from 4 Singaporean-Chinese individuals (S4 cohort). We also performed RNA-Seq and microarray transcriptome profiling of PBMCs from an extended cohort of 13 individuals (S13 cohort). The data was used first to characterize the transcriptomic signatures and relationships among the 29 immune cell types. Then we explored the difference in mRNA composition in terms of tra...

Provenance — who produced it, who reused it

Linked to 26 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Gianni MonacoBernett LeeWeili XuYou HwangMichael PoidingerJoão de MagalhãesAnis Larbi
Reused by

20 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
127 / 127 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 227708435900 reported
total reads 2277084359 reported
n content pct 0.003 measured
pct q20 bases 97.9 measured
pct q30 bases 93 measured
gc content pct 46.2 measured
mean read length 50 measured
mean base quality 36.1 measured
adapter content pct 0.01 measured
duplication rate pct 21.28 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93 measured ×1 100%
mean base quality 36.1 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 21.28 measured ×0.4 100%
QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0