Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE107313

GEO first seen 2018

Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function

Organism
Homo sapiens
Samples
6
Type
Expression profiling by high...
Submitted
2017-11-24

VCP is an evolutionary conserved ubiquitin-dependent ATPase that mediates the degradation of proteins through the ubiquitin-proteasome pathway. Despite the central role of VCP in the regulation of protein homeostasis, identity and nature of its cellular substrates remain poorly defined. Here, we combined chemical inhibition of VCP and quantitative ubiquitin remnant profiling to assess the effect of VCP inhibition on the ubiquitin-modified proteome and to probe the substrate spectrum of VCP in hu...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Petra Beli
Reused by

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

NextSeq 500 short-read RNA-seq from human samples with 152M reads and moderate quality metrics (88.7% Q30); typical of NextSeq chemistry trade-offs between depth and base quality—addresses standard transcriptomics questions like differential expression or isoform detection.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
6 / 6 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 24209776773 reported
total reads 152262747 reported
n content pct 0 measured
pct q20 bases 94.2 measured
pct q30 bases 88.7 measured
gc content pct 49.8 measured
mean read length 159 measured
mean base quality 33.5 measured
adapter content pct 34.74 measured
duplication rate pct 17.12 measured
supplementary file types CSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 88.7 measured ×1 94%
mean base quality 33.5 measured ×0.6 92%
adapter content pct 34.74 measured ×0.4 0%
duplication rate pct 17.12 measured ×0.4 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0