Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE107644

GEO first seen 2018

Single-cell RNA-seq Uncovers Dynamic Processes and Critical Regulators in Mouse Spermatogenesis

Organism
Mus musculus
Samples
106
Type
Expression profiling by high...
Submitted
2017-12-04

A systematic interrogation of male germ cells is key to complete understanding of molecular mechanisms governing spermatogenesis and the development of new strategies for infertility therapies and male contraception. Here we develop an approach to purify all types of homogeneous spermatogenic cells by combining transgenic labelling and synchronization of the cycle of the seminiferous epithelium, and subsequent single-cell RNA-sequencing. We reveal extensive and previously uncharacterized dynamic...

Provenance — who produced it, who reused it

Linked to 9 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Yao ChenYuxuan ZhengYun GaoZhen LinSuming YangTongtong WangQiu WangNannan XieRong HuaMingxi LiuJiahao ShaMichael D. GriswoldJinsong LiFuchou TangMing-Han Tong
Reused by

6 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Instrument
HiSeq X Ten
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
106 / 106 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 1157199790128 reported
total reads 3856672553 reported
n content pct 0.004 measured
pct q20 bases 93.1 measured
pct q30 bases 85.3 measured
gc content pct 44.7 measured
mean read length 150 measured
mean base quality 36.2 measured
adapter content pct 1.06 measured
duplication rate pct 51.84 measured
supplementary file types BEDGRAPH, TAB, TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.3 measured ×1 77%
QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0