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Single cell profilng of hCS derived from hiPSC cultured in feeder-free conditions
Single cell gene expression profilling of hCS derived from iPSC at day 105 of differentiation
Provenance — who produced it, who reused it
Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deposited / produced by
Sergiu PascaChristina Fan
Reused by
- Reliability of human cortical organoid generation 2018 · 512 cites
- Long-term maturation of human cortical organoids matches key ear... 2021 · 358 cites
Deep data QC
65/100 · DStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
6 / 3 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok
yes
reported
total bases
107248580736
reported
total reads
530933568
reported
n content pct
0.5
measured
pct q20 bases
87.5
measured
pct q30 bases
79.4
measured
gc content pct
41.9
measured
mean read length
101
measured
mean base quality
32.8
measured
adapter content pct
3.39
measured
duplication rate pct
46.91
measured
supplementary file types
CSV
reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 65/100
The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
pct q30 bases
79.4
measured
×1
47%
mean base quality
32.8
measured
×0.6
80%
adapter content pct
3.39
measured
×0.4
92%
duplication rate pct
46.91
measured
×0.4
62%
QC cost
19 s compute
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0