Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE107874

GEO first seen 2018

A tetraspanin family member functionally resolves and facilitates the purification of adult pluripotent stem cells used for whole-body regeneration (Bulk)

Organism
Schmidtea mediterranea
Samples
96
Type
Expression profiling by high...
Submitted
2017-12-08

Proliferating cells known as neoblasts include pluripotent stem cells (PSCs) capable of sustaining tissue homeostasis and regeneration of lost body parts in planarians. However, the lack of markers to prospectively identify and isolate these adult PSCs has significantly hampered their characterization. We used single-cell RNA sequencing (scRNA-seq) and single cell transplantation to address this long-standing issue. Large-scale scRNA-seq of fluorescently sorted neoblasts unveiled a novel subtype...

Provenance — who produced it, who reused it

Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
An ZengHua LiAlejandro Sánchez Alvarado
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Schmidtea mediterranea
Metrics (value · how obtained)
n content pct 0.008 measured
pct q20 bases 97.7 measured
pct q30 bases 95.3 measured
gc content pct 37.1 measured
mean read length 51 measured
mean base quality 35.6 measured
adapter content pct 0 measured
duplication rate pct 47.48 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.3 measured ×1 100%
mean base quality 35.6 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 47.48 measured ×0.4 61%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0