Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE108097

GEO first seen 2018

Mapping Mouse Cell Atlas by Microwell-seq

Organism
Homo sapiens; Mus musculus
Samples
90
Type
Expression profiling by high...
Submitted
2017-12-14

We developed Microwell-seq, a high-throughput and low-cost scRNA-seq platform using simple, inexpensive devices. Using Microwell-seq, we constructed a basic scheme for the Mouse Cell Atlas. We reveal single cell hierarchy for many tissues that have not been well previously characterized. Our study demonstrates the wide applicability of the Microwell-seq technology and the Mouse Cell Atlas resource.

Provenance — who produced it, who reused it

Linked to 21 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Xiaoping HanRenying Wang
Reused by

12 further papers cite this accession but reuse could not be confirmed.

Deep data QC

81/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens; Mus musculus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
178 / 90 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 3166657704485 reported
total reads 14813828729 reported
n content pct 0.006 measured
pct q20 bases 95.1 measured
pct q30 bases 89.1 measured
gc content pct 40.7 measured
mean read length 117.6 measured
mean base quality 37.4 measured
adapter content pct 0 measured
duplication rate pct 78.08 measured
supplementary file types TAR, TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 81/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.1 measured ×1 96%
mean base quality 37.4 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 78.08 measured ×0.4 0%
QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0