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mRNA sequencing to assess RfxCasR and matching shRNA specificity
Matching sets of RfxCasR and shRNAs targeting ANXA4 and B4GALNT1 plus non-targeting (NT) controls were profiled by mRNA sequencing to compare non-specific transcriptome perturbations for both shRNA and RfxCasR technologies.
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deposited / produced by
Patrick HsuMaxim N ShokhirevSilvana Konermann
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
21 / 21 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok
yes
reported
total bases
27036224376
reported
total reads
321859814
reported
supplementary file types
TXT
reported
QC cost
5 s compute
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently