Next Generation Sequencing Facilitates Comparative Transcriptomes Analysis for Saccharomyces cerevisiae lines generated by SCRaMbLE
Purpose: According to comparative transcriptomes data, the differentially expressed genes within these selected scrambled yeast strains can be digged out and further analyzed, seeking potential targets that were tuned drastically and might improve β-carotene production when modified. Methods: mRNA profiles of two Saccharomyces cerevisiae strains H0, S3 (each named as yJBH000 and yJBH012 in related manuscript) were generated in triplicate using illumina. Sequencing libraries were generated using...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Precise control of SCRaMbLE in synthetic haploid and diploid yea... 2018 · 162 cites
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
No quantitative QC rubric exists for this data type yet, so it is deliberately left unscored — this is an honest "not applicable", not a poor rating.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently