Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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GSE108890

GEO first seen 2019

Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032

Organism
Zymomonas mobilis subsp. mobilis
Samples
17
Type
Expression profiling by high...
Submitted
2018-01-08

In this study, we reported the updated chromosome and plasmid sequence of ZM4, and its two engineered xylose-utilizing strains derivatives (strains 2032 and 8b). The majority of plasmid genes have either homologs from other organisms or some conserved domains. Our bioinformatics analysis suggested that several plasmid genes may be essential genes of ZM4. To further validate the function of these plasmid genes, an RNA-Seq pipeline was developed for Z. mobilis and used to compare the gene express...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Shihui YangJessica M VeraJeff GrassGiannis SavvakisOleg MoskvinYongfu YangSean J McIlwainYucai LyuIrene ZinonosAlexaner S HebertJoshua J CoonDonna M BatesTrey K SatoSteven D BrownMichael E HimmelMin ZhangRobert LandickKatherine PappasYaoping Zhang
Reused by

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Zymomonas mobilis (ethanologenic bacterium) with unavailable platform details (HTTP error), but exceptional quality metrics (97.2% Q30, 99.8 bp read length, 0% N content). This dataset captures bacterial transcriptomics relevant to biofuel production and industrial fermentation. Researchers should contact GEO to obtain sequencing platform and to verify the long read length for a bacterial RNA-seq experiment.

Data type / assay
bulk-RNA-seq
Organism
Zymomonas mobilis subsp. mobilis
Metrics (value · how obtained)
n content pct 0 measured
pct q20 bases 98.8 measured
pct q30 bases 97.2 measured
gc content pct 50.8 measured
mean read length 99.8 measured
mean base quality 37.1 measured
adapter content pct 0 measured
duplication rate pct 70.55 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.2 measured ×1 100%
mean base quality 37.1 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 70.55 measured ×0.4 10%
QC cost 35 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0