GSE109485

GEO

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mouse bulk-RNA-seq on HiSeq 2500 with 305 million reads at 97.2% Q20 and 94% Q30 provides good quality for transcript profiling. The reported short-read type and reasonable coverage support sensitive expression quantification and isoform detection in murine tissues.

Data type / assay

bulk-RNA-seq

Organism

Mus musculus

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

12 / 12 runs

Completeness

100%

Metrics (value · how obtained)

checksum ok yes reported

total bases 15233832650 reported

total reads 304676653 reported

n content pct 0.003 measured

pct q20 bases 97.2 measured

pct q30 bases 94 measured

gc content pct 51.5 measured

mean read length 50 measured

mean base quality 36.2 measured

adapter content pct 0 measured

duplication rate pct 19.24 measured

supplementary file types TXT reported

How this grade was computed

Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94 measured ×1 100%

mean base quality 36.2 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 19.24 measured ×0.4 100%

QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0