Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE109816

GEO first seen 2020

Dissecting cell composition and cell-cell interaction network of normal human heart tissue by single-cell sequencing

Organism
Homo sapiens
Samples
12
Type
Expression profiling by high...
Submitted
2018-01-29

We studied the cell compositon of normal human heart by single-cell sequencing. Distint subgroups of cardiac muscle, fibroblast cell and endothelial cell were detected. We drawed a cell-cell interaction network using specific expressed ligands and receptors of cells. And we also observed the change of interaction and cell transformation with age.

Provenance — who produced it, who reused it

Linked to 14 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Li WangPeng YuZheng LiZongna Ren
Reused by

12 further papers cite this accession but reuse could not be confirmed.

Deep data QC

85/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.068 measured
pct q20 bases 93.9 measured
pct q30 bases 92.1 measured
gc content pct 42.9 measured
mean read length 25.7 measured
mean base quality 33.5 measured
adapter content pct 0 measured
duplication rate pct 64.37 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 85/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.1 measured ×1 100%
mean base quality 33.5 measured ×0.6 92%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 64.37 measured ×0.4 24%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0