Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE109974

GEO first seen 2019

RNA polymerase II activity revealed by GRO-seq and pNET-seq in Arabidopsis

Organism
Arabidopsis thaliana
Samples
12
Type
Expression profiling by high...
Submitted
2018-02-01

RNA polymerase II (Pol II) play an essential role in gene expression. Here, we adapted mammalian Native Elongation Transcript sequencing and Global Run On sequencing to profile nascent RNA genome wide in Arabidopsis. We found Pol II tends to accumulate downstream of transcription start site and pausing at proximal promoter is an important regulatory step for Pol II transcription although loosely controlled. Furthermore, the Pol II with unphosphorylated carboxyl-terminal domain (CTD) mainly accum...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Jiafu ZhuMin LiuXiaobin LiuZhicheng Dong
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

metadata only · no data-level QC for this type

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
other
Organism
Arabidopsis thaliana
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
12 / 12 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 58935926180 reported
total reads 611626124 reported
supplementary file types BEDGRAPH, TXT reported
QC cost 7 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently