Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE110993

GEO first seen 2021

RNA-seq identifies circulating miR-125a-5p, miR-125b-5p and miR-143-3p as potential biomarkers for acute ischemic stroke

Organism
Homo sapiens
Samples
40
Type
Non-coding RNA profiling by...
Submitted
2018-02-22

Rationale: Currently, there are no blood-based biomarkers with clinical utility for acute ischemic stroke (IS). microRNAs (miRNAs) show promise as disease markers due to their cell-type specific expression patterns and stability in peripheral blood. Objective: To identify circulating miRNAs associated with acute IS, determine their temporal course up to 90 days post-stroke, and explore their utility as an early diagnostic marker. Methods and Results: We used RNA sequencing to study expression ch...

Provenance — who produced it, who reused it

Linked to 10 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Bernd H NorthoffLesca M HoldtKnut Krohn
Reused by

9 further papers cite this accession but reuse could not be confirmed.

Deep data QC

82/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.108 measured
pct q20 bases 93.7 measured
pct q30 bases 89.3 measured
gc content pct 52.1 measured
mean read length 51 measured
mean base quality 36.4 measured
adapter content pct 0 measured
duplication rate pct 87.27 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 82/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 89.3 measured ×1 97%
mean base quality 36.4 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 87.27 measured ×0.4 0%
QC cost 27 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0