Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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GSE111478

GEO first seen 2018

miRNA data from extracellular vesicles secreted by Nippostrongylus brasiliensis

Organism
Nippostrongylus brasiliensis
Samples
3
Type
Non-coding RNA profiling by...
Submitted
2018-03-06

miRNA-Seq analysis of the miRNA present in the extracellular vesicles secreted by the nematode Nippostrongylus brasiliensis

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Ramon M EichenbergerMatt FieldJavier SotilloAlex Loukas

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Nippostrongylus brasiliensis (parasitic nematode) with unavailable sequencing platform (HTTP error), but concerning quality metrics show very high N content (5.384%) indicating potential contamination or sequencing artifacts. This dataset captures parasite transcriptomics but the severe data quality issues require aggressive filtering before reliable transcript quantification. Researchers should contact GEO to investigate the underlying cause of high N content.

Data type / assay
bulk-RNA-seq
Organism
Nippostrongylus brasiliensis
Metrics (value · how obtained)
n content pct 5.384 measured
pct q20 bases 90 measured
pct q30 bases 87.8 measured
gc content pct 47.9 measured
mean read length 55 measured
mean base quality 32.3 measured
adapter content pct 0.99 measured
duplication rate pct 44.24 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 87.8 measured ×1 89%
mean base quality 32.3 measured ×0.6 72%
adapter content pct 0.99 measured ×0.4 100%
duplication rate pct 44.24 measured ×0.4 68%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0