Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE111586

GEO first seen 2018

A Single-Cell Atlas of In Vivo Mammalian Chromatin Accessibility

Organism
Mus musculus
Samples
17
Type
Genome binding/occupancy pro...
Submitted
2018-03-08

We applied a combinatorial indexing assay, sci-ATAC-seq, to profile genome-wide chromatin accessibility in ~100,000 single cells from 13 adult mouse tissues. We identify 85 distinct patterns of chromatin accessibility, most of which can be assigned to cell types, and ~400,000 differentially accessible elements. We use these data to link regulatory elements to their target genes, to define the transcription factor grammar specifying each cell type, and to discover in vivo correlates of heterogene...

Provenance — who produced it, who reused it

Linked to 17 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Darren A CusanovichAndrew J HillDelasa AghamirzaieRiza M DazaHannah A PlinerJoel B BerletchGalina N FilippovaXingfan HuangLena ChristiansenWilliam S DeWittCholi LeeSamuel G RegaladoDavid F ReadFrank J SteemersChristine M DistecheCole TrapnellJay Shendure
Reused by

14 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ATAC-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
34 / 17 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 346357443800 reported
total reads 3463574438 reported
n content pct 0.013 measured
pct q20 bases 100 measured
pct q30 bases 100 measured
gc content pct 49.1 measured
mean read length 50 measured
mean base quality 30 measured
adapter content pct 0.06 measured
duplication rate pct 4.52 measured
supplementary file types TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ATAC-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 100 measured ×1 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0