Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE112066

GEO first seen 2018

Regulation of follicular T-helper cell positioning and function by PD-1

Organism
Mus musculus
Samples
12
Type
Expression profiling by high...
Submitted
2018-03-20

Follicular T-helper (TFH) cells highly express the programmed cell death-1 (PD-1) molecule. Whereas inhibition of T cell receptor (TCR) signaling and CD28 co-stimulation is thought to be the primary mode of PD-1 functions, how PD-1 regulates TFH development and function remains unclear. Here we show that, when engaged by the ensemble of bystander B cells constitutively expressing PD-1 ligand 1 (PD-L1), PD-1 inhibits T-cell recruitment into follicles. This inhibition involves suppression of PI3K...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Hai QiJingwen ShiShiyue HouQian FangXin LiuXiaolong Liu

Deep data QC

58/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Mus musculus with unavailable instrument details (HTTP fetch error), but quality metrics show moderate base quality (78.6% Q30, 76 bp read length, 0.053% N content). This dataset enables basic mouse transcriptomics but the limited quality and unknown platform prevent optimal data evaluation. Researchers should contact GEO for complete experimental metadata before interpreting results.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.053 measured
pct q20 bases 86.2 measured
pct q30 bases 78.6 measured
gc content pct 37.8 measured
mean read length 76 measured
mean base quality 32.2 measured
adapter content pct 2.29 measured
duplication rate pct 58.9 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 58/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.6 measured ×1 43%
mean base quality 32.2 measured ×0.6 70%
adapter content pct 2.29 measured ×0.4 98%
duplication rate pct 58.9 measured ×0.4 36%
QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0