Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE112368

GEO first seen 2019

Transcriptome analysis using RNA sequencing conducted for wild type (Col-0) and ahl10-1 mutant under control conditions and after 96 h at moderate low water potential (-0.7 MPa)

Organism
Arabidopsis thaliana
Samples
12
Type
Expression profiling by high...
Submitted
2018-03-27

RNA sequencing was used to identify genes differentially expressed (DEG) in ahl10-1 mutant compared to Col-0 wild type. Two treatments were analyzed for each genotype: unstressed control and a 96 hour low water potential (-0.7 MPa) treatment. P<0.05 and fold change ≥ 1.25 used as cut off value for DEG. Approximately 2000-3000 genes were found to be significantly up and down-regulated in the wild type stress versus control treatment using . In control treated seedlings, 10 or less than 10 genes a...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Paul VersluesMin M Wong
Reused by

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Arabidopsis thaliana with unavailable instrument information (HTTP fetch error), but excellent quality metrics (93.7% Q30, 151 bp read length). This dataset enables plant gene expression profiling in a model organism, though complete platform specifications require contacting GEO. The long read length suggests paired-end sequencing suitable for isoform detection.

Data type / assay
bulk-RNA-seq
Organism
Arabidopsis thaliana
Metrics (value · how obtained)
n content pct 0.003 measured
pct q20 bases 96.7 measured
pct q30 bases 93.7 measured
gc content pct 45.6 measured
mean read length 151 measured
mean base quality 35.5 measured
adapter content pct 24.12 measured
duplication rate pct 22.68 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.7 measured ×1 100%
mean base quality 35.5 measured ×0.6 100%
adapter content pct 24.12 measured ×0.4 0%
duplication rate pct 22.68 measured ×0.4 100%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0