Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE112509

GEO first seen 2021

RNA-seq analysis identifies different transcriptomic types and developmental trajectories of primary melanomas

Organism
Homo sapiens
Samples
80
Type
Expression profiling by high...
Submitted
2018-03-29

Recent studies revealed trajectories of mutational events in early melanomagenesis, but the accompanying changes in gene expression are far less understood. Therefore, we performed a comprehensive RNA-Seq analysis of laser-microdissected melanocytic nevi (n=23) and primary melanoma samples (n=57) and characterized the molecular mechanisms of early melanoma development. Using self-organizing maps, unsupervised clustering and analysis of pseudotime (PT) dynamics to identify evolutionary trajectori...

Provenance — who produced it, who reused it

Linked to 8 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Manfred KunzHenry Löffler-WirthMichael DannemannEdith WillscherGero DooseJanet KelsoTina KottekBirgit NickelLydia HoppJenny LandsbergSteve HoffmannThomas TütingPaola ZigrinoCornelia MauchJochen UtikalMirjana ZiemerHans-Joachim SchulzeMichael HölzelAlexander RoeschSusanne KneitzSvenja MeierjohannAnja BosserhoffHans BinderManfred Schartl
Reused by

7 further papers cite this accession but reuse could not be confirmed.

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.132 measured
pct q20 bases 98.4 measured
pct q30 bases 97.7 measured
gc content pct 45.3 measured
mean read length 64.9 measured
mean base quality 57.8 measured
adapter content pct 9.51 measured
duplication rate pct 49.73 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.7 measured ×1 100%
mean base quality 57.8 measured ×0.6 100%
adapter content pct 9.51 measured ×0.4 58%
duplication rate pct 49.73 measured ×0.4 56%
QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0