Chromatin Remodeler RSC2 Modulates Binding of the Transcriptional Activator Ace1p and Improves the Transcription of CUP1 by Fast Cycles of Remodeling
Little is known about how dynamic binding of Transcription Factors (TF) is coordinated with chromatin remodeling. Using Single Molecule Tracking (SMT) we show that a chromatin remodeler (RSC2 complex) speeds up the search process of the TF Ace1p for its Response Elements (RE) in CUP1 promoter, ensuring a homogeneous response of the cell population to heavy metal stress. By SMT and MNase-seq, RSC2 binds to activated promoter transiently indicating that preinitiation at CUP1 is dependent on short...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deep data QC
insufficient data to scoreStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0