Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE113230

GEO first seen 2018

High-throughput RNA sequencing on circular RNA profiles of human triple-negative breast cancer and adjacent normal tissues

Organism
Homo sapiens
Samples
6
Type
Expression profiling by high...
Submitted
2018-04-16

In an attempt to search for metastasis-associated circRNAs, we performed RNA-sequencing on ribosomal RNA-depleted total RNA from three pairs of triple-negative breast cancer (TNBC) and adjacent normal tissues. A computational pipeline based on the anchor alignment of unmapped reads was used to identify circular RNAs. Taken together, 69,815 distinct circRNAs were found in this study and 87% were derived from exons, and the others were derived from introns, intergenic region and 3′ or 5′ UTR, etc....

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Kaixuan ZengShukui Wang
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.005 measured
pct q20 bases 97.4 measured
pct q30 bases 93.6 measured
gc content pct 60.1 measured
mean read length 150 measured
mean base quality 38.7 measured
adapter content pct 8.4 measured
duplication rate pct 66.65 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.6 measured ×1 100%
mean base quality 38.7 measured ×0.6 100%
adapter content pct 8.4 measured ×0.4 64%
duplication rate pct 66.65 measured ×0.4 19%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0