Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE114007

GEO first seen 2019

Identification of transcription factors responsible for dysregulated networks in human osteoarthritis cartilage by global gene expression analysis

Organism
Homo sapiens
Samples
38
Type
Expression profiling by high...
Submitted
2018-05-03

Objective:Osteoarthritis (OA) is the most prevalent joint disease. As disease-modifying therapies are not available, novel therapeutic targets need to be discovered and prioritized for their importance in mediating the abnormal phenotype of cells in OA-affected joints. Here, we generated a genome-wide molecular profile of OA to elucidate regulatory mechanisms of OA pathogenesis and to identify possible therapeutic targets using integrative analysis of mRNA-sequencing data obtained from human kne...

Provenance — who produced it, who reused it

Linked to 40 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Kathleen M FischRamya GaminiOscar Alvarez-GarciaRyuichiro AkagiMasahiko SaitoYuta MuramatsuTakahisa SashoAndrew I SuMartin K Lotz
Reused by

38 further papers cite this accession but reuse could not be confirmed.

Deep data QC

63/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
38 / 38 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 64419597774 reported
total reads 753892984 reported
n content pct 0.026 measured
pct q20 bases 87.1 measured
pct q30 bases 81.6 measured
gc content pct 46.6 measured
mean read length 100 measured
mean base quality 32 measured
adapter content pct 12.1 measured
duplication rate pct 34.88 measured
supplementary file types TXT, XLSX reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 63/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.6 measured ×1 58%
mean base quality 32 measured ×0.6 67%
adapter content pct 12.1 measured ×0.4 44%
duplication rate pct 34.88 measured ×0.4 89%
QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0