Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE114235

GEO first seen 2018

Exosome release is regulated by mTORC1 via a Rab27A-dependent mechanism

Organism
Homo sapiens
Samples
2
Type
Non-coding RNA profiling by...
Submitted
2018-05-09

Exosomes are small membrane-bound vesicles released into extracellular spaces by many types of cells. These nanovesicles carry proteins, mRNA and miRNA and are involved in cell waste management and intercellular communication. In the present study we show that exosome release, which leads to net loss of cellular membrane and protein content, is negatively regulated by mechanistic target of rapamycin complex 1 (mTORC1). We find that in cells and animal models exosome release is inhibited by susta...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Wenchong ZouXiaochun Bai

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

miRNA-seq of Homo sapiens via Illumina Genome Analyzer with exceptional quality (97.6% Q30, 0.004% N content) and confirmed GEO UID. This dataset enables human microRNA discovery and quantification across tissues or disease states. The excellent base quality and mature miRNA-appropriate read length (~50 bp) support robust microRNA identification and target validation studies.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina Genome Analyzer
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
2 / 2 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 1898468900 reported
total reads 37969378 reported
n content pct 0.004 measured
pct q20 bases 99.1 measured
pct q30 bases 97.6 measured
gc content pct 56 measured
mean read length 50 measured
mean base quality 39.5 measured
adapter content pct 94.89 measured
duplication rate pct 81.59 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.6 measured ×1 100%
mean base quality 39.5 measured ×0.6 100%
adapter content pct 94.89 measured ×0.4 0%
duplication rate pct 81.59 measured ×0.4 0%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0