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Single cell RNA seq for Ciona late tailbud embryos
We applied single cell RNA seq with 10x genomics platform to study the cell identity change in Ciona late tailbud embryos. By comparing single cell data from Pax3/7>Foxc amd control embryos, we found ectopic expression of FoxC in BTN cells transformed into PSC cells, which suggest common shared origin of BTNs and PSCs.
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deposited / produced by
Kai Chen
Reused by
- Shared evolutionary origin of vertebrate neural crest and crania... 2018 · 122 cites
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
Data type / assay
bulk-RNA-seq
Organism
Ciona intestinalis
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
BAM/CRAM (aligned)
N numbers (samples, groups)
2 / 2 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok
no
reported
total bases
18391682875
reported
total reads
147133463
reported
supplementary file types
CLOUPE, H5
reported
QC cost
6 s compute
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently