Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE115478

GEO first seen 2021

Drosophila melanogaster late third instar larval testes and gonadal fatbody RNA-seq profiling

Organism
Drosophila melanogaster
Samples
24
Type
Expression profiling by high...
Submitted
2018-06-07

In order to study the effect of protease treatment on dissected testes, we performed different strategies of cleaning and dissociation on Drosophila melanogaster w1118 larval testes. We collected testes without protease treatment with fatbody just attached around the gonad, fatbody alone dissected from around the testes, testes without fatbody and testes dissociated by either by Papain or Trypsin and Collagenase cocktail. We prepared poly A+ RNA-Seq libraries and performed transcriptional profil...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Sharvani MahadevarajuJustin M FearBrian Oliver
Reused by

Deep data QC

metadata only · no data-level QC for this type

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
bulk-RNA-seq
Organism
Drosophila melanogaster
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
24 / 24 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 7696295862 reported
total reads 150907762 reported
supplementary file types BIGWIG, GTF, TXT reported
QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently