Single Cell sequencing of E14.5, E15.5 and E14.5 Kir electroporated cortical neurons.
Experimental in vivo hyperpolarization of E14.5 apical progenitors shifted their transcriptional programs with precocious generation of intermediate progenitors, and a forward shift in the laminar, molecular, morphological and circuit features of their neuronal progeny. In particular, we reported an overall repression of L4-type neuronal genes and a premature induction of L2/3-type neuronal genes following Kir2.1 overexpression.
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Progenitor Hyperpolarization Regulates the Sequential Generation... 2018 · 161 cites
Deep data QC
86/100 · BStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Bulk RNA-seq of Mus musculus with unavailable instrument details but good quality metrics (94% Q30, 70 bp read length, 0.059% N content). This dataset enables mouse transcriptomics studies, though the unknown platform limits optimization of data processing pipelines. Contacting GEO is recommended to obtain the specific Illumina instrument used for this sequencing.
The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0