Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE116583

GEO first seen 2018

Alveolar Macrophages after Murine Lung Transplant

Organism
Mus musculus
Samples
12
Type
Expression profiling by high...
Submitted
2018-07-03

The objective of this study was to examine transcriptional changes in alveolar macrophages early after reperfusion in a model of murine lung transplant.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Stephen F Chiu
Reused by

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Mus musculus via NextSeq 500 with good base quality (94.1% Q30, 0.012% N content) and confirmed GEO UID. This dataset supports mouse gene expression studies with a mid-throughput platform enabling moderate multiplex efficiency. The consistent quality metrics enable reliable transcript quantification and differential expression analysis.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
12 / 12 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 20776188257 reported
total reads 275487766 reported
n content pct 0.012 measured
pct q20 bases 96.1 measured
pct q30 bases 94.1 measured
gc content pct 49.2 measured
mean read length 75.4 measured
mean base quality 34.4 measured
adapter content pct 0 measured
duplication rate pct 14.82 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.1 measured ×1 100%
mean base quality 34.4 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 14.82 measured ×0.4 100%
QC cost 10 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0