Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE117176

GEO first seen 2019

Single cell RNA sequencing of murine bone marrow derived macrophages and adipose tissue macrophages from lean and obese mice

Organism
Mus musculus
Samples
5
Type
Expression profiling by high...
Submitted
2018-07-16

Macrophages are key to innate immunity and tissue homeostasis. Plasticity of macrophages allows rapid and highly orchestrated responses to various pathogenic and physiological challenges. Capturing the dynamic intracellular programs employed by macrophages during encounters with various stimuli presents a demanding technical challenge, and failures to fully characterize some of these responses has stalled development of disease-specific therapy. Powered by single-cell RNA sequencing (scRNA-seq),...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Chuan LiBeiyan Zhou
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), BAM/CRAM (aligned)
N numbers (samples, groups)
5 / 5 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 182326644864 reported
total reads 1860475968 reported
n content pct 0.016 measured
pct q20 bases 93.6 measured
pct q30 bases 82.4 measured
gc content pct 42.3 measured
mean read length 98 measured
mean base quality 35.5 measured
adapter content pct 0 measured
duplication rate pct 80.55 measured
supplementary file types MTX, TSV reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.4 measured ×1 62%
mean base quality 35.5 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 80.55 measured ×0.4 0%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0