High-throughput single-cell ChIP-seq identifies heterogeneity of chromatin states in breast cancer
The dynamic nature of chromatin and transcription plays a critical role in normal differentiation and is expected to contribute to tumor evolution. Studying chromatin heterogeneity with single-cell resolution is mandatory to understand the role of epigenetic plasticity in cancer. Here, we describe a droplet microfluidics system that enables the profiling of chromatin marks of individual cells with an average coverage of up to 10,000 loci per cell. In patient-derived xenograft (PDX) models of bre...
Provenance — who produced it, who reused it
Linked to 5 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Joint profiling of histone modifications and transcriptome in si... 2021 · 314 cites
4 further papers cite this accession but reuse could not be confirmed.
Deep data QC
insufficient data to scoreStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0