Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE117993

GEO first seen 2023

Transcription profiles of rectal biopsies obtained during diagnostic colonoscopy for pediatric inflammatory bowel diseases.

Organism
Homo sapiens
Samples
190
Type
Expression profiling by high...
Submitted
2018-08-01

RNA was isolated from rectal biopsies from 190 pediatric patients undergoing diagnostic colonoscopy for inflammatory bowel diseases, including Crohn's disease and ulcerative colitis. Single-end, 75-bp sequencing was performed, and raw reads aligned to the human genome using Gencode v 24 as reference. We included 14085 protein-coding mRNA genes in downstream analyses, where cutoffs of fold change>1.5 and FDR<0.05 were considered significant.

Provenance — who produced it, who reused it

Linked to 13 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Rebekah KarnsYael HabermanTed Denson
Reused by

12 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.016 measured
pct q20 bases 94.9 measured
pct q30 bases 91.7 measured
gc content pct 50.7 measured
mean read length 75 measured
mean base quality 36.2 measured
adapter content pct 0.23 measured
duplication rate pct 27.38 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.7 measured ×1 100%
mean base quality 36.2 measured ×0.6 100%
adapter content pct 0.23 measured ×0.4 100%
duplication rate pct 27.38 measured ×0.4 100%
QC cost 39 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0