Alarmin S100A11 initiates a chemokine response to the human pathogen Toxoplasma gondii
Purpose: The goal of this study is to compare NGS-derived transcriptome profiling of human PBMC infected with T. gondii, PRU and RH strains, (RNA-seq) to not stimulated human PBMC transcriptome (RNA-seq). Methods: After 24h post infection PBMC and non-infected PBMC mRNA profiles were generated by deep sequencing. The sequence reads were analyzed within Galaxy platform: FastQC(*) program was used to generate quality control reports followed by employment of Trimmamatic (*) to trim “noisy” ends;...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deep data QC
99/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Bulk RNA-seq of Homo sapiens with unavailable platform information (HTTP error), but good quality metrics (93.7% Q30, 79.2 bp read length, 0.006% N content). This dataset enables human gene expression studies with intermediate-length reads suitable for isoform identification. Researchers should contact GEO for complete sequencing platform specifications.
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0