Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE121182

GEO first seen 2019

Dynamic behavior of the epigenetic machinery upon thermal stress

Organism
Homo sapiens
Samples
4
Type
Genome binding/occupancy pro...
Submitted
2018-10-12

Maintenance of the epigenome is of utmost importance to warrant appropriate cellular functioning Here we analyzed PRC1/2 function, in response to cellular stress. We observe that heat shock (HS) leads to nucleolar accumulation of CBX proteins, and are strongly immobilized in a 1,6-hexanediol insensitive manner, suggestive of a HS-induced liquid-to-solid phase transition of the nucleolus. CBX protein recovery from the nucleolus is dependent on heat shock protein (HSP) activity. LC-MS/MS analyses...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Maria AzkanazAida R LópezBauke de BoerPierre-Olivier AngrandEdo VellengaHarm H KampingaSteven BerginkJoost H MartensJan J SchuringaVincent van den Boom
Reused by

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.008 measured
pct q20 bases 97.3 measured
pct q30 bases 96 measured
gc content pct 43.3 measured
mean read length 42 measured
mean base quality 34.6 measured
adapter content pct 0 measured
duplication rate pct 4.08 measured
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96 measured ×1 100%
QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0