Transcriptome Analysis of CircMRPS35 mediated mRNA expression profiles
Purpose: To explore the mechanism of circMRPS35-mediated suppression of proliferation and invasion in gastric cancer. Methods: MGC-803 cells were transfected with control and SiRNA-circMRPS35, in triplicate. Total RNA was extracted using Trizol reagent.
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deep data QC
100/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Bulk RNA-seq of Homo sapiens with unavailable instrument details (HTTP error), but good quality (94% Q30, 150 bp read length, 2.49% adapter content) and confirmed GEO UID. This dataset enables human transcriptomics with long paired-end reads suitable for isoform resolution. The moderate adapter content suggests incomplete adapter trimming, requiring verification before downstream use.
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0