Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE12241

GEO first seen 2012

Genome-wide maps of chromatin state in pluripotent and lineage-committed cells

Organism
Mus musculus
Samples
21
Type
Genome binding/occupancy pro...
Submitted
2008-07-24

Genome-wide maps of chromatin state (H3K4me3, H3K9me3, H3K27me3, H3K36me3, H4K20me3) in pluripotent and lineage-committed cells We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mammalian cells. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of mouse embryonic stem cells, neural progenitor cells and embryonic fibroblasts. We find...

Provenance — who produced it, who reused it

Linked to 33 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Tarjei S MikkelsenManching KuEric S LanderBradley E Bernstein
Reused by

22 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Instrument
Illumina Genome Analyzer
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
54 / 21 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 13644616274 reported
total reads 443685182 reported
n content pct 5.705 measured
pct q20 bases 57.8 measured
pct q30 bases 49.2 measured
gc content pct 39.8 measured
mean read length 27 measured
mean base quality 20.3 measured
adapter content pct 0 measured
duplication rate pct 10.39 measured
supplementary file types TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 49.2 measured ×1 0%
QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0