Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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GSE123441

GEO first seen 2020

MBNL1 regulates essential alternative RNA splicing patterns in MLL-rearranged leukemia

Organism
Homo sapiens
Samples
28
Type
Expression profiling by high...
Submitted
2018-12-06

In this study, we identify MBNL1 as a unique molecular vulnerability across MLL-rearranged leukemias. Through transcriptomic profiling and novel splicing analyses of MLL-rearranged leukemia cell lines following shRNA knockdown of MBNL1, we show that MBNL1 regulates alternative splicing (predominantly intron retention) of genes essential to MLL-rearranged leukemogenesis, such as DOT1L and SETD1A.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
S S ItskovichA GurunathanJ ClarkM BurwinkelM WunderlichM R BergerA KulkarniK ChetalM VenkatasubramanianN SalomonisA R KumarL H Lee
Reused by

Deep data QC

99/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Homo sapiens with unavailable instrument details (HTTP error), but excellent quality (95.5% Q30, 75 bp read length, 0.006% N content). This dataset enables human transcriptomics with high-throughput short reads. The minimal contamination and consistent quality support reliable differential expression analysis across samples.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.006 measured
pct q20 bases 97.3 measured
pct q30 bases 95.5 measured
gc content pct 50.7 measured
mean read length 75 measured
mean base quality 38.2 measured
adapter content pct 0.01 measured
duplication rate pct 32.29 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 99/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.5 measured ×1 100%
mean base quality 38.2 measured ×0.6 100%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 32.29 measured ×0.4 95%
QC cost 23 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0