Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE124263

GEO first seen 2020

Neonatal and adult human testis defined at the single-cell level

Organism
Homo sapiens
Samples
8
Type
Expression profiling by high...
Submitted
2018-12-21

Spermatogenesis has been well studied in rodents and invertebrates, but remains poorly understood in humans. As a step towards illuminating human spermatogenesis, we used single-cell RNA-sequencing (scRNAseq) analysis to analyze neonatal and adult human testes. Clustering analysis of neonatal testes revealed 3 germ subsets, including cells with characteristics of primordial germ cells (PGCs), and more differentiated cells with gene expression profiles similar with adult spermatogonial stem cells...

Provenance — who produced it, who reused it

Linked to 8 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Abhishek SohniKun TanHye-Won SongDana BurowMiles F Wilkinson
Reused by

5 further papers cite this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 4000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
128 / 8 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 117901534920 reported
total reads 950818830 reported
n content pct 0.035 measured
pct q20 bases 99.1 measured
pct q30 bases 96.9 measured
gc content pct 50.4 measured
mean read length 26 measured
mean base quality 38.8 measured
adapter content pct 0 measured
duplication rate pct 5.7 measured
supplementary file types MTX, TSV, XLSX reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.9 measured ×1 100%
mean base quality 38.8 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 5.7 measured ×0.4 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0