Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE125065

GEO first seen 2019

Single cell transcriptomic analysis of the lateral hypothalamic area reveals molecularly distinct populations of inhibitory and excitatory neurons

Organism
Mus musculus
Samples
2
Type
Expression profiling by high...
Submitted
2019-01-14

We employed a droplet-based single cell RNA-sequencing (scRNA-seq) approach to develop a comprehensive census of molecularly distinct cell types in the mouse lateral hypothalamic area (LHA). In addition to 13 distinct non-neuronal cell populations, we define 15 distinct populations of glutamatergic and 15 distinct populations of GABAergic neurons, including both known and novel cell types. This comprehensive transcriptomic analysis of LHA cell types lays the groundwork for understanding the circ...

Provenance — who produced it, who reused it

Linked to 5 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Alexander JacksonPaul Robson
Reused by

4 further papers cite this accession but reuse could not be confirmed.

Deep data QC

74/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.008 measured
pct q20 bases 90.6 measured
pct q30 bases 78.7 measured
gc content pct 44.6 measured
mean read length 98 measured
mean base quality 34.6 measured
adapter content pct 0.02 measured
duplication rate pct 36.45 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 74/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 78.7 measured ×1 44%
mean base quality 34.6 measured ×0.6 100%
adapter content pct 0.02 measured ×0.4 100%
duplication rate pct 36.45 measured ×0.4 86%
QC cost 41 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0