Pioneer and nonpioneer factor cooperation drives lineage specific chromatin opening [scRNA-seq]
Pioneer transcription factors are coined as having the unique property of “opening closed chromatin sites” for implementation of cell fates. We previously showed that the pioneer Pax7 specifies melanotrope cells through deployment of an enhancer repertoire: this allows binding of Tpit, a nonpioneer factor that determines the related lineages of melanotropes and corticotropes. Here, we investigated the relation between these two factors in the pioneer mechanism. Cell-specific gene expression and...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deep data QC
44/100 · FStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Bulk RNA-seq of Mus musculus with unavailable platform information (HTTP error), but concerning quality metrics showing very low Q30 (64.4%), poor Q20 (74.5%), and long read length (100 bp). This dataset captures mouse transcriptomics but severe quality issues compromise reliability. Researchers should contact GEO to investigate underlying sequencing or data processing problems before proceeding with analysis.
The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0