Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE125693

GEO first seen 2019

RNA-seq analysis of MPCs treated with FGF1 variants

Organism
Mus musculus
Samples
6
Type
Expression profiling by high...
Submitted
2019-01-25

RNA-seq is a powerful tool to analyze differential expression of cellular pathways under different conditions. The goal of this study is to analyze the potential pathways involved in cellular defense against high glucose challenge with or without FGF1 intervention.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Dezhong WangZhifeng Huang
Reused by

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This murine bulk RNA-seq dataset exhibits high read quality (95.7% Q30, 98.5% Q20) with 150.8 bp reads and negligible N content, indicating clean library preparation and sequencing. No adapter contamination detected, suggesting minimal post-sequence processing artifacts. While the specific sequencing instrument is not recorded, the quality metrics indicate suitability for transcript quantification and expression profiling in mouse models.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.007 measured
pct q20 bases 98.5 measured
pct q30 bases 95.7 measured
gc content pct 49 measured
mean read length 150.8 measured
mean base quality 39.4 measured
adapter content pct 0 measured
duplication rate pct 29.65 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.7 measured ×1 100%
mean base quality 39.4 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 29.65 measured ×0.4 100%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0