Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE125742

GEO

Provenance — who produced it, who reused it

Linked to 0 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

No linked papers found in the corpus yet.

Deep data QC

30/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Mouse RNA-seq with 150 bp reads achieved only 70.5% Q20 and 56.2% Q30 with unusually low 20.5% GC—anomalous for a mammalian transcriptome. The poor base quality (29.1 mean) and strange nucleotide composition suggest contamination, sequencing error, or sample swap; dataset quality is highly questionable without validation.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0 measured
pct q20 bases 70.5 measured
pct q30 bases 56.2 measured
gc content pct 20.5 measured
mean read length 150 measured
mean base quality 29.1 measured
adapter content pct 0.02 measured
duplication rate pct 50.43 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 30/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 56.2 measured ×1 0%
mean base quality 29.1 measured ×0.6 18%
adapter content pct 0.02 measured ×0.4 100%
duplication rate pct 50.43 measured ×0.4 55%

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 94