Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE126030

GEO first seen 2022

A single cell reference map for human blood and tissue T cell activation

Organism
Homo sapiens
Samples
16
Type
Expression profiling by high...
Submitted
2019-02-04

Human T cells coordinate adaptive immunity by localization in diverse tissue sites, though blood T cells are the most readily studied. We investigated the functional responses of T cells isolated from human lungs (LG), lymph nodes (LN), bone marrow (BM), and blood to TCR-stimulation using single-cell RNA-seq. We defined cellular states for resting T cells including signatures that differentiate tissue and blood T cells and employed new factorization methods to identify activation states conserv...

Provenance — who produced it, who reused it

Linked to 3 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Peter A SzaboHanna Mendes LevitinMichelle MironMark E SnyderTakashi SendaJinzhou YuanYim L ChengErin C BushPranay DograPuspa ThapaDonna L FarberPeter A Sims
Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

86/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.067 measured
pct q20 bases 99.7 measured
pct q30 bases 99.1 measured
gc content pct 50.2 measured
mean read length 26 measured
mean base quality 39.5 measured
adapter content pct 0 measured
duplication rate pct 69.11 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 86/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 99.1 measured ×1 100%
mean base quality 39.5 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 69.11 measured ×0.4 13%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0