Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE126579

GEO first seen 2019

Inferring population dynamics from single-cell RNA-sequencing time-series data

Organism
Mus musculus
Samples
1
Type
Expression profiling by high...
Submitted
2019-02-14

This dataset consists of single-cell RNA-seq (Drop-seq) data from thymi of day 14.5 mouse embryos. The sample includes the whole thymus, including mesenchyme, endothelium, epithelium, thymocytes, and other lymphocytes. The mouse is a Rag2-/- knockout.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
David S FischerAnna K FiedlerEric M KernfeldRyan M GengaAimee Bastidas-PonceMostafa BakhtiHeiko LickertJan HasenauerRene MaehrFabian Theis
Reused by

Deep data QC

28/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

This murine bulk RNA-seq dataset is compromised for most downstream analyses due to poor sequence quality (59.3% Q30, 60.6% Q20), very short 20 bp reads, and high N-content (35.4%), combined with a low mean base quality of 22.1. These metrics indicate either problematic sequencing conditions or degraded input material; reuse should be approached cautiously and may require aggressive quality filtering.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 35.419 measured
pct q20 bases 60.6 measured
pct q30 bases 59.3 measured
gc content pct 33.3 measured
mean read length 20 measured
mean base quality 22.1 measured
adapter content pct 0 measured
duplication rate pct 44.5 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 28/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 59.3 measured ×1 0%
mean base quality 22.1 measured ×0.6 0%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 44.5 measured ×0.4 68%
QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0