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GSE128815

GEO first seen 2019

Analysis of mRNA expression changes caused by mdt-15(tm2182) mutation at 15oC and 25 oC

Organism
Caenorhabditis elegans
Samples
12
Type
Expression profiling by high...
Submitted
2019-03-25

To determine the role of MDT-15 in mRNA expression changes at different temperatures, we sequenced mRNAs obtained from day 1 adult Caenorhabditis elegans. Bristol N2 (wild-type) and mdt-15(tm2182) mutant animals that cultured at 25oC or 15oC were used.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Seung-Jae V LeeDongyeop LeeSeon-Woo A AnYoonji Jung
Reused by

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq of Caenorhabditis elegans with unavailable sequencing platform (HTTP error), but exceptional quality metrics (95.4% Q30, 101 bp read length, 0.003% N content). This dataset supports nematode transcriptomics in a model developmental organism. The long reads and high quality enable resolution of C. elegans developmental isoforms.

Data type / assay
bulk-RNA-seq
Organism
Caenorhabditis elegans
Metrics (value · how obtained)
n content pct 0.003 measured
pct q20 bases 98.5 measured
pct q30 bases 95.4 measured
gc content pct 48.4 measured
mean read length 101 measured
mean base quality 39.1 measured
adapter content pct 0.52 measured
duplication rate pct 36.08 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.4 measured ×1 100%
mean base quality 39.1 measured ×0.6 100%
adapter content pct 0.52 measured ×0.4 100%
duplication rate pct 36.08 measured ×0.4 87%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0