Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE129785

GEO first seen 2019

Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion

Organism
Homo sapiens; Mus musculus
Samples
67
Type
Genome binding/occupancy pro...
Submitted
2019-04-14

Understanding complex tissues requires single-cell deconstruction of gene regulation with precision and scale. Here we present a massively parallel droplet-based platform for mapping transposase-accessible chromatin in tens of thousands of single cells per sample (scATAC-seq). We obtain and analyze chromatin profiles of over 200,000 single cells in two primary human systems. In blood, scATAC-seq allows marker-free identification of cell type-specific cis- and trans-regulatory elements, mapping o...

Provenance — who produced it, who reused it

Linked to 23 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Jeff GranjaGrace ZhengPreyas Shah
Reused by

14 further papers cite this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ATAC-seq
Organism
Homo sapiens; Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
67 / 67 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 1934408083892 reported
total reads 18032920882 reported
n content pct 0.077 measured
pct q20 bases 83.5 measured
pct q30 bases 74.6 measured
gc content pct 48.5 measured
mean read length 33 measured
mean base quality 31.6 measured
adapter content pct 0 measured
duplication rate pct 65.73 measured
supplementary file types MTX, TSV, TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ATAC-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 74.6 measured ×1 23%
QC cost 5 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0